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XB-GRNA-23659135
gRNA Name:
celsr2 gRNA1
Synonyms: celsr2_g1
Genomic Target: celsr2
PAM Sequence: TGG
Sequence: 5' GTGGCTGTAATTCTGGCCAG 3'
Synonyms: celsr2_g1
Genomic Target: celsr2
PAM Sequence: TGG
Sequence: 5' GTGGCTGTAATTCTGGCCAG 3'
Genomic Target | tropicalis | laevis.L | |||||
Identities | 20/20 | 20/20 | |||||
genome
gRNA
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5' CTGGCCAGAATTACAGCCAC 3' |||||||||||||||||||| 3' GACCGGTCTTAATGTCGGTG 5' |
5' CTGGCCAGAATTACAGCCAC 3' |||||||||||||||||||| 3' GACCGGTCTTAATGTCGGTG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 10.1 | Chr2L:73229125..73229144 | 20/20 | Antisense | target | celsr2.L | ||
Chr3L:83868854..83868873 | 17/20 | Antisense | off-target | slc26a5.L | |||
Chr6S:39434449..39434468 | 16/20 | Antisense | off-target | thrb.S | |||
Chr2S:53103365..53103384 | 16/20 | Antisense | off-target | LOC108709434 | |||
Chr5S:115781780..115781799 | 18/20 | Sense | unknown | tropicalis 10.0 | Chr2:63373489..63373508 | 20/20 | Sense | target | celsr2 |
Chr7:102363479..102363498 | 17/20 | Antisense | unknown | ||||
Chr8:86684606..86684625 | 16/20 | Sense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020 |
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???morpholino.displaySummary.papersRecent??? |
Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022 |
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???morpholino.displaySummary.papersAll??? |