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Summary Attributions Wiki
XB-GRNA-23659139
gRNA Name: itsn1 gRNA1
Synonyms: itsn1_g1
Genomic Target: itsn1

PAM Sequence: AGG
Sequence: 5' GCAGGAACTTGCGTGGGCCC 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 19/20
genome
gRNA
 
5' GGGCCCACGCAAGTTCCTGC 3'
   ||||||||||||||||||||
3' CCCGGGTGCGTTCAAGGACG 5'
 
5' GGGCCCACGCAAGTTCCTGC 3'
   ||||||||||||||||||||
3' CCCGGGTGCGTTCAAGGACG 5'
 
5' GGGCCCACGCAAGTTCCTAC 3'
   |||||||||||||||||| |
3' CCCGGGTGCGTTCAAGGACG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:13718175..13718194 20/20 Sense target itsn1.L
Chr2S:20258255..20258274 19/20 Antisense target itsn1.S
Chr3L:75066976..75066995 16/20 Antisense unknown
Chr9_10S:27668820..27668839 16/20 Antisense unknown
tropicalis 10.0 Chr2:14218325..14218344 20/20 Antisense target itsn1
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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