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Summary Attributions Wiki
XB-GRNA-23659141
gRNA Name: nog gRNA1
Synonyms:
Genomic Target: nog

PAM Sequence: AGG
Sequence: 5' GGAGGAGAGACTTGGAGTGG 3'



Genomic Target tropicalis laevis.L
Identities 20/20 19/20
genome
gRNA
 
5' CCACTCCAAGTCTCTCCTCC 3'
   ||||||||||||||||||||
3' GGTGAGGTTCAGAGAGGAGG 5'
 
5' CCACTCCAAGTCTCTCCTCT 3'
   ||||||||||||||||||| 
3' GGTGAGGTTCAGAGAGGAGG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr9_10L:27176019..27176038 19/20 Sense target nog.L
chr9_10S:43702169..43702188 16/20 Sense off-target Xelaev18046953m
Scaffold71115:61..80 19/20 Sense unknown
chr1S:103882137..103882156 17/20 Sense unknown
chr9_10L:103053138..103053157 17/20 Antisense unknown
chr4S:17583197..17583216 16/20 Sense unknown
chr9_10L:42401966..42401985 16/20 Sense unknown
tropicalis 10.0 Chr10:25898904..25898923 20/20 Sense target nog
Chr2:147329874..147329893 18/20 Sense off-target rarg
Chr5:23873284..23873303 17/20 Antisense off-target c2orf73
Chr8:147092862..147092881 17/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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