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Summary Attributions Wiki
XB-GRNA-23659165
gRNA Name: fxr1 gRNA4
Synonyms: T7L fxr1
Genomic Target: fxr1

PAM Sequence: TGG
Sequence: 5' ACTGCAGGTTGCAAACATATTGG 3'



Genomic Target laevis.L
Identities 23/23
genome
gRNA
 
5' CCAATATGTTTGCAACCTGCAGT 3'
   |||||||||||||||||||||||
3' GGTTATACAAACGTTGGACGTCA 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr5L:115244358..115244380 23/23 Antisense target fxr1.L
Chr6L:68032436..68032458 21/23 Sense off-target eepd1.L
Chr6L:115358572..115358594 20/23 Sense off-target rock1.L
Chr7L:95158703..95158725 20/23 Sense off-target prkcz.L
Chr9_10L:84682214..84682236 20/23 Sense off-target spag16.L
Chr9_10L:84682214..84682236 20/23 Sense off-target LOC108701425
Chr9_10S:59923220..59923242 19/23 Antisense off-target neb.S
Chr8L:24178351..24178373 19/23 Sense off-target pappa.L
Chr8L:24175617..24175639 19/23 Sense off-target pappa.L
Chr3L:110432552..110432574 19/23 Sense off-target trpm7.L
Chr4L:240677..240699 19/23 Sense off-target tsg101.L
Chr7S:6786042..6786064 19/23 Antisense off-target pcdh15.S
Chr9_10L:89572299..89572321 19/23 Antisense off-target nbeal1.L
Chr3L:142714409..142714431 19/23 Antisense off-target LOC121401618
Chr3L:142756179..142756201 19/23 Antisense off-target LOC108710639
Chr9_10L:31525130..31525152 21/23 Antisense unknown
Chr2S:107224459..107224481 21/23 Antisense unknown
Chr6L:48308799..48308821 20/23 Antisense unknown
Chr2L:113269154..113269176 20/23 Sense unknown
Chr3L:142817282..142817304 19/23 Antisense unknown
Chr6L:54562..54584 19/23 Sense unknown
Chr1S:128161658..128161680 19/23 Antisense unknown
Chr8S:80576090..80576112 19/23 Sense unknown
Chr9_10L:130205526..130205548 19/23 Sense unknown
tropicalis 10.0 Chr6:36256846..36256868 19/23 Sense off-target etv1
Chr1:184943894..184943916 19/23 Antisense off-target crhbp
Chr3:104816036..104816058 19/23 Sense off-target trpm7
Chr6:94613256..94613278 19/23 Sense off-target cdkal1
Chr1:121123987..121124009 19/23 Sense unknown
Chr6:116815119..116815141 19/23 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells., J Cell Biol 2020     
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