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Summary Attributions Wiki
XB-GRNA-23659167
gRNA Name: fxr1 gRNA6
Synonyms: T8L fxr1
Genomic Target: fxr1

PAM Sequence: TGG
Sequence: 5' TCAGGACAATGGTTCTTAAATGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 22/23 23/23 20/23
genome
gRNA
 
5' CCATTTAAGAACCATTGTCTTGA 3'
   ||||||||||||||||||| |||
3' GGTAAATTCTTGGTAACAGGACT 5'
 
5' CCATTTAAGAACCATTGTCCTGA 3'
   |||||||||||||||||||||||
3' GGTAAATTCTTGGTAACAGGACT 5'
 
5' CCATTTCAGAACCATTGTCTTTA 3'
   |||||| |||||||||||| | |
3' GGTAAATTCTTGGTAACAGGACT 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr5L:115244015..115244037 23/23 Antisense target fxr1.L
Chr5S:94960479..94960501 20/23 Antisense target fxr1.S
Chr1L:192613188..192613210 21/23 Sense off-target skic3.L
Chr8S:29905659..29905681 21/23 Sense off-target col5a1.S
Chr1S:68850541..68850563 20/23 Sense off-target btc.S
Chr6L:77637692..77637714 20/23 Sense off-target LOC108719059
Chr1L:15692674..15692696 19/23 Sense off-target ctnna2.L
Chr1S:7792583..7792605 19/23 Sense off-target ctnna2.S
Chr2S:128953321..128953343 19/23 Sense off-target ankrd52.S
Chr4L:119866346..119866368 19/23 Antisense off-target rangap1.L
Chr5L:77937764..77937786 19/23 Sense off-target gprc6a.2.L
Chr7L:134062502..134062524 20/23 Sense unknown
Chr8S:6560331..6560353 20/23 Sense unknown
Chr5L:9758632..9758654 20/23 Sense unknown
Chr2S:148660601..148660623 19/23 Antisense unknown
Chr3S:69157295..69157317 19/23 Sense unknown
Chr5L:34862641..34862663 19/23 Sense unknown
Chr5L:38538402..38538424 19/23 Sense unknown
Chr7S:31728421..31728443 19/23 Antisense unknown
Chr7S:64209341..64209363 19/23 Antisense unknown
Chr8S:13821779..13821801 19/23 Sense unknown
Chr1S:91859558..91859580 19/23 Antisense unknown
tropicalis 10.0 Chr5:108318945..108318967 22/23 Antisense target fxr1
Chr1:58511030..58511052 20/23 Antisense off-target znf827
Chr9:49725024..49725046 19/23 Antisense off-target ccnyl1
Chr1:127000230..127000252 20/23 Sense unknown
Chr6:114240556..114240578 19/23 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells., J Cell Biol 2020     
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