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Summary Attributions Wiki
XB-GRNA-23659193
gRNA Name: fgfr4 gRNA3
Synonyms: CRISPR-3
Genomic Target: fgfr4

PAM Sequence: TGG
Sequence: 5' TGCAGGGGAATACACATGTCTGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 23/23 22/23
genome
gRNA
 
5' CCAGACATGTGTATTCCCCTGCA 3'
   |||||||||||||||||||||||
3' GGTCTGTACACATAAGGGGACGT 5'
 
5' CCAGACATGTGTATTCCCCTGCA 3'
   |||||||||||||||||||||||
3' GGTCTGTACACATAAGGGGACGT 5'
 
5' CCAGACATGTGTACTCCCCTGCA 3'
   ||||||||||||| |||||||||
3' GGTCTGTACACATAAGGGGACGT 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr3L:39275994..39276016 23/23 Antisense target fgfr4.L
chr3S:98899640..98899662 22/23 Sense target fgfr4.S
chr6L:25426430..25426452 20/23 Sense off-target mrc1.L
chr6L:6585691..6585713 20/23 Sense off-target zbtb47.L
chr3L:65183166..65183188 19/23 Antisense off-target cerk.L
chr9_10S:37656029..37656051 19/23 Antisense off-target cox10.S
chr7L:109738732..109738754 19/23 Antisense off-target LOC108697046
chr6S:20972436..20972458 19/23 Sense off-target LOC108719870 [provisional:mrc1]
chr7L:83941914..83941936 19/23 Sense off-target LOC108696538 [provisional:plch2]
chr6L:122655910..122655932 20/23 Antisense unknown
chr7L:12433013..12433035 20/23 Sense unknown
chr1S:102821056..102821078 19/23 Antisense unknown
chr8L:81436798..81436820 19/23 Antisense unknown
chr9_10L:73243300..73243322 19/23 Sense unknown
tropicalis 10.0 Chr3:46325203..46325225 23/23 Antisense target fgfr4
Chr2:45639449..45639471 20/23 Sense off-target rilp
Chr2:126257038..126257060 19/23 Sense off-target cln5
Chr2:74615587..74615609 19/23 Antisense off-target parl
Chr6:28345267..28345289 19/23 Sense off-target mrc1
Chr6:13464531..13464553 19/23 Sense off-target scn5a
Chr7:90227896..90227918 19/23 Sense off-target plch2
Chr7:108097819..108097841 19/23 Antisense off-target LOC105947618
Chr7:108099252..108099274 19/23 Antisense off-target LOC105947618
Chr7:108100305..108100327 19/23 Antisense off-target LOC105947618
Chr7:111653600..111653622 20/23 Antisense unknown
Chr2:71890719..71890741 19/23 Antisense unknown
Chr2:77297406..77297428 19/23 Antisense unknown
Chr3:69016797..69016819 19/23 Antisense unknown
Chr6:74545994..74546016 19/23 Sense unknown
Chr6:74549007..74549029 19/23 Sense unknown
Chr6:74577478..74577500 19/23 Antisense unknown
Chr7:108079918..108079940 19/23 Sense unknown
Chr7:108081064..108081086 19/23 Sense unknown
Chr7:108082496..108082518 19/23 Sense unknown
Chr7:88569801..88569823 19/23 Antisense unknown
Chr8:104429130..104429152 19/23 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus., Front Physiol 2018     
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