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Summary Attributions Wiki
XB-GRNA-23659206
gRNA Name: sox9 gRNA1
Synonyms:
Genomic Target: sox9

PAM Sequence: AGG
Sequence: 5' GGAGCTGAAGAAGGAGACAG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 19/20 19/20
genome
gRNA
 
5' CTGTCTCCTTCTTCAGCTCC 3'
   ||||||||||||||||||||
3' GACAGAGGAAGAAGTCGAGG 5'
 
5' CTGTCTCCTTCTTCATCTCC 3'
   ||||||||||||||| ||||
3' GACAGAGGAAGAAGTCGAGG 5'
 
5' CCGTCTCCTTCTTCAGCTCC 3'
   | ||||||||||||||||||
3' GACAGAGGAAGAAGTCGAGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 Scaffold30:1783331..1783350 19/20 Sense target sox9.L
chr9_10S:19079771..19079790 19/20 Sense target sox9.S
chr1L:53153609..53153628 19/20 Antisense off-target rnf150.L
chr1L:53153609..53153628 19/20 Antisense off-target Xelaev18005544m
chr4S:113294829..113294848 18/20 Antisense off-target rybp.S
chr1L:10168356..10168375 18/20 Sense off-target ptcd3.L
chr8S:85088692..85088711 18/20 Sense off-target myh13.S
Scaffold24:3084110..3084129 18/20 Sense off-target ptpdc1.L
Scaffold24:3084192..3084211 18/20 Sense off-target ptpdc1.L
chr2S:159491315..159491334 18/20 Sense off-target arfip2.S
chr4L:45881815..45881834 18/20 Sense off-target LOC108714047
chr1L:105507126..105507145 18/20 Antisense off-target LOC108713377 [provisional:shoc1]
chr2S:58893541..58893560 17/20 Antisense off-target nucks1.S
chr8L:572498..572517 17/20 Antisense off-target myh1-2-1.L
chr8L:555297..555316 17/20 Antisense off-target LOC108698090 [provisional:myh4]
chr1L:86704332..86704351 17/20 Sense off-target LOC108711853 [provisional:ptprs]
chr3L:19796680..19796699 16/20 Antisense off-target ik.L
Scaffold47:762911..762930 19/20 Antisense unknown
chr5S:56905562..56905581 19/20 Antisense unknown
chr1S:73636482..73636501 18/20 Antisense unknown
chr8L:17121415..17121434 18/20 Antisense unknown
chr1S:166777147..166777166 17/20 Antisense unknown
chr2L:5363432..5363451 17/20 Antisense unknown
chr3S:83443288..83443307 17/20 Antisense unknown
chr7S:61036013..61036032 17/20 Antisense unknown
Scaffold23:3221224..3221243 16/20 Antisense unknown
chr7L:11685974..11685993 16/20 Antisense unknown
tropicalis 10.0 Chr10:30592353..30592372 20/20 Sense target sox9
Chr3:126118760..126118779 19/20 Sense off-target camk2b
Chr2:138886296..138886315 18/20 Antisense off-target LOC100170425
Chr7:120185353..120185372 18/20 Sense off-target LOC116412249
Chr8:749673..749692 17/20 Antisense off-target myh2
Chr8:749673..749692 17/20 Antisense off-target tbc1d25
Chr8:770776..770795 17/20 Antisense off-target LOC101730386
Chr3:22973947..22973966 16/20 Antisense off-target ik
Chr2:138236687..138236706 16/20 Antisense off-target dnajc14
Chr6:122641028..122641047 16/20 Sense off-target LOC100495973
Chr1:46405464..46405483 19/20 Sense unknown
Chr2:18819127..18819146 19/20 Antisense unknown
Chr4:72958412..72958431 19/20 Sense unknown
Chr5:160418769..160418788 19/20 Sense unknown
Chr8:89635971..89635990 19/20 Antisense unknown
Chr1:42943012..42943031 17/20 Sense unknown
Chr3:80438108..80438127 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos., Cell Biosci 2016     
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