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XB-GRNA-23659701
gRNA Name:
cela1.6 gRNA2
Synonyms: elastase -T2
Genomic Target: cela1.6
PAM Sequence: AGG
Sequence: 5' GGTGGTGATGGAGTCCGTTC 3'
Synonyms: elastase -T2
Genomic Target: cela1.6
PAM Sequence: AGG
Sequence: 5' GGTGGTGATGGAGTCCGTTC 3'
Genomic Target | tropicalis | ||||||
Identities | 20/20 | ||||||
genome
gRNA
|
5' GAACGGACTCCATCACCACC 3' |||||||||||||||||||| 3' CTTGCCTGAGGTAGTGGTGG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 10.1 | Chr2L:150032284..150032303 | 20/20 | Antisense | off-target | cela1.3.L | ||
Chr2L:150017199..150017218 | 19/20 | Antisense | off-target | cela1.4.L | |||
Chr2L:150043791..150043810 | 19/20 | Antisense | off-target | cela1.2.L | |||
Chr9_10L:129578651..129578670 | 18/20 | Antisense | unknown | ||||
Chr9_10L:130421478..130421497 | 18/20 | Sense | unknown | ||||
Chr7S:42437525..42437544 | 17/20 | Antisense | unknown | ||||
Chr7L:59871941..59871960 | 16/20 | Sense | unknown | tropicalis 10.0 | Chr2:142386711..142386730 | 20/20 | Antisense | target | cela1.6 |
Chr2:142375768..142375787 | 20/20 | Antisense | off-target | cela1.4 | |||
Chr2:142398836..142398855 | 20/20 | Antisense | off-target | cela1.2 | |||
Chr4:11011669..11011688 | 16/20 | Antisense | off-target | slc1a2 | |||
Chr4:11011690..11011709 | 16/20 | Antisense | off-target | slc1a2 | |||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014 |
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???morpholino.displaySummary.papersRecent??? | |||||||
???morpholino.displaySummary.papersAll??? |