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XB-GRNA-23659710
gRNA Name:
pgat gRNA3
Synonyms: pat-T3
Genomic Target: pgat
PAM Sequence: tgg
Sequence: 5' GGCAGCTCTTGGAAGAACAG 3'
Synonyms: pat-T3
Genomic Target: pgat
PAM Sequence: tgg
Sequence: 5' GGCAGCTCTTGGAAGAACAG 3'
Genomic Target | tropicalis | ||||||
Identities | 20/20 | ||||||
genome
gRNA
|
5' CTGTTCTTCCAAGAGCTGCC 3' |||||||||||||||||||| 3' GACAAGAAGGTTCTCGACGG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr5S:71784474..71784493 | 18/20 | Antisense | off-target | bckdhb.S | ||
chr2S:145776910..145776929 | 17/20 | Antisense | off-target | cntn5.S | |||
chr9_10L:2205075..2205094 | 17/20 | Antisense | off-target | pgap3.L | |||
chr7L:122234271..122234290 | 16/20 | Sense | off-target | LOC108696880 [provisional:kmt2b] | |||
chr7S:87583982..87584001 | 16/20 | Sense | off-target | LOC108697287 [provisional:kmt2b] | |||
chr5S:26926076..26926095 | 18/20 | Antisense | unknown | ||||
chr7S:45724216..45724235 | 16/20 | Sense | unknown | tropicalis 10.0 | Chr5:138707102..138707121 | 20/20 | Sense | target | pgat |
Chr5:143605430..143605449 | 17/20 | Sense | off-target | ldah | |||
Chr10:9904702..9904721 | 17/20 | Antisense | off-target | pgap3 | |||
Chr7:72490734..72490753 | 17/20 | Antisense | off-target | arhgap32 | |||
Chr7:128945272..128945291 | 16/20 | Antisense | off-target | kmt2b | |||
Chr7:70982013..70982032 | 16/20 | Antisense | off-target | kirrel3 | |||
Chr3:114189005..114189024 | 16/20 | Sense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014 |
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???morpholino.displaySummary.papersRecent??? | |||||||
???morpholino.displaySummary.papersAll??? |