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XB-GRNA-23659711
gRNA Name:
pdx1 gRNA1
Synonyms: pdx1-T1
Genomic Target: pdx1
PAM Sequence: tgg
Sequence: 5' GGGCCAGCATTTCATTTTCT 3'
Synonyms: pdx1-T1
Genomic Target: pdx1
PAM Sequence: tgg
Sequence: 5' GGGCCAGCATTTCATTTTCT 3'
Genomic Target | tropicalis | ||||||
Identities | 20/20 | ||||||
genome
gRNA
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5' AGAAAATGAAATGCTGGCCC 3' |||||||||||||||||||| 3' TCTTTTACTTTACGACCGGG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr1S:16625926..16625945 | 18/20 | Antisense | off-target | stk32b.S | ||
chr1L:124206417..124206436 | 17/20 | Sense | off-target | ptprd.L | |||
chr1S:130816979..130816998 | 17/20 | Antisense | off-target | Xetrov90003056m.S | |||
chr3L:60792871..60792890 | 16/20 | Antisense | off-target | cped1.L | |||
chr4L:44611953..44611972 | 16/20 | Antisense | off-target | vat1l.L | |||
chr5L:86533955..86533974 | 16/20 | Sense | off-target | bach2.L | |||
chr3L:60792871..60792890 | 16/20 | Antisense | off-target | LOC108710266 [provisional:cped1] | |||
chr2S:107501804..107501823 | 18/20 | Sense | unknown | ||||
chr4S:99040227..99040246 | 18/20 | Sense | unknown | ||||
chr6S:72419707..72419726 | 18/20 | Antisense | unknown | ||||
chr2L:129500220..129500239 | 17/20 | Antisense | unknown | ||||
chr1L:169712047..169712066 | 16/20 | Sense | unknown | ||||
chr2L:18962513..18962532 | 16/20 | Antisense | unknown | ||||
chr4S:90299075..90299094 | 16/20 | Antisense | unknown | ||||
chr5L:114989782..114989801 | 16/20 | Sense | unknown | ||||
chr6S:105025285..105025304 | 16/20 | Antisense | unknown | tropicalis 10.0 | Chr2:155135614..155135633 | 20/20 | Sense | target | pdx1 |
Chr2:118100820..118100839 | 17/20 | Antisense | off-target | fam155a | |||
Chr1:60157398..60157417 | 16/20 | Antisense | off-target | il15 | |||
Chr4:103977984..103978003 | 16/20 | Antisense | off-target | bcar3 | |||
Chr8:67589114..67589133 | 16/20 | Sense | off-target | mbnl3 | |||
Chr2:114154689..114154708 | 16/20 | Sense | off-target | gabrg3 | |||
Chr1:213535050..213535069 | 16/20 | Sense | off-target | LOC100490503 | |||
Chr1:139239351..139239370 | 18/20 | Antisense | unknown | ||||
Chr10:36153581..36153600 | 17/20 | Antisense | unknown | ||||
Chr4:123446052..123446071 | 17/20 | Sense | unknown | ||||
Chr8:91798069..91798088 | 17/20 | Antisense | unknown | ||||
Chr3:102627525..102627544 | 16/20 | Sense | unknown | ||||
Chr5:88037053..88037072 | 16/20 | Sense | unknown | ||||
Chr8:79280029..79280048 | 16/20 | Antisense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014 |
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???morpholino.displaySummary.papersRecent??? | |||||||
???morpholino.displaySummary.papersAll??? |