Summary Attributions

Name: pGEM®-11Zf(–)	Type: plasmid	Supplier: Promega
Description: The pGEM®-11Zf(+) and pGEM®-11Zf(–) Vectors can be used as standard cloning vectors, as templates for in vitro transcription and for the production of ssDNA. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109).
Sequence: [+] 1 GGGCGAATTG GCCAAGTCGG CCGAGCTCGA ATTCGTCGAC CTCGAGGGAT51 CCGGGCCCTC TAGATGCGGC CGCATGCATA A GCTTGAGTA TTCTATAGTG101 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA151 ATTGTTATCC GCT CACAATT CCACACAACA TACGAGCCGG AAGCATAAAG201 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 251 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT301 AATGAATCGG CCAACGCGCG GGGAGAGGCG G TTTGCGTAT TGGGCGCTCT351 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG401 AGCGGTATCA GCT CACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG451 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC551 TGACGAGCAT CACAAAAATC GACGCTCAAG T CAGAGGTGG CGAAACCCGA601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC651 TCTCCTGTTC CGA CCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 751 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC T ATCGTCTTG AGTCCAACCC851 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA901 GCAGAGCGAG GTA TGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT951 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA1051 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT G CAAGCAGCA GATTACGCGC1101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA1151 CGCTCAGTGG AAC GAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT1201 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 1251 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT1301 AATCAGTGAG GCACCTATCT CAGCGATCTG T CTATTTCGT TCATCCATAG1351 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA1401 TCTGGCCCCA GTG CTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC1451 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 1501 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA1551 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT T TGCGCAACG TTGTTGCCAT1601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA1651 GCTCCGGTTC CCA ACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC1701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 1751 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA1801 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG T GACTGGTGA GTACTCAACC1851 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC1901 GTCAATACGG GAT AATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA1951 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 2001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC2051 ATCTTTTACT TTCACCAGCG TTTCTGGGTG A GCAAAAACA GGAAGGCAAA2101 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA2151 CTCTTCCTTT TTC AATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT2201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 2251 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT2301 ATCATGACAT TAACCTATAA AAATAGGCGT A TCACGAGGC CCTTTCGTCT2351 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG2401 AGACGGTCAC AGC TTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT2451 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 2501 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC2551 CGCACAGATG CGTAAGGAGA AAATACCGCA T CAGGACGCG CCCTGTAGCG2601 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA2651 CTTGCCAGCG CCC TAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT2701 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 2751 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT2801 TAGGGTGATG GTTCACGTAG TGGGCCATCG C CCTGATAGA CGGTTTTTCG2851 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA2901 CTGGAACAAC ACT CAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG2951 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 3001 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TCCATTCGCC3051 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG A TCGGTGCGG GCCTCTTCGC3101 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG3151 GTAACGCCAG GGT TTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA3201 ATTGTAATAC GACTCACTAT A

Antibiotic resistance: Ampicillin	Promoter:
Reporter Gene:	Gene Tag: