Larger Image FIG. 2. XtDSCAM is expressed in the nervous system during early development. A: RTPCR analysis of individual Xenopus embryos at different stages in development using primers specific for XtDSCAM and XtGAPDH. Results are shown for single embryos in triplicate. BâD: In situ hybridization staining of neurula (B, Stage 19), early tailbud (C, Stage 22), and tadpole stage (D, Stage 27) X. tropicalis embryos using an XtDSCAM-specific probe. Arrows point to areas with significant DSCAM staining, Nc, notochord; Hb, hindbrain; Op, olfactory placode; Tn, trigeminal nerve; Ep, epibranchial placodes. Scalebar5100 mm. Image published in: Morales Diaz HD (2014) Copyright © 2014. Image reproduced with permission of the Publisher.
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