Larger Image FIGURE 5 Distinct, but partially overlapping expression patterns of calretinin/S100Z. (a) In premetamorphic X. laevis, ORNs across the whole MOE expressed calretinin (magenta) and S100Z (yellow). Not all but many cells showed co-localization of the two calcium-binding proteins (e.g., white dotted circles). (b) In premetamorphic animals, the axonal projection of calretinin-expressing neurons (magenta) targeted the AOB, LC, and MC. Within the LC, calretinin- and S100Z-positive (yellow) glomeruli were detected and some were double-positive (white dotted ovals). (c) Expression patterns of calretinin (magenta) and S100Z (yellow) in glomeruli of the lateral cluster in four different premetamorphic animals. The expression patterns were very variable between individuals, but some trends for recurring patterns were apparent. The outer lateral glomeruli within the LC had the tendency to be either S100Z- or calretinin-positive (asterisks), whereas in the medial part of the LC, more glomeruli were S100Z-positive. The most caudal glomerulus in the middle of the LC was often double positive for S100Z/calretinin (arrows). (d) Immunohistochemical staining for calretinin (magenta) labeled neurons throughout the whole VNO in premetamorphic animals, but no S100Z expression (yellow) was found in the VNO. A anterior; AOB, accessory olfactory bulb; L, lateral; LC, lateral cluster; M, medial; MC, medial cluster; MOE, main olfactory epithelium; ORN, olfactory receptor neuron; P, posterior; VNO, vomeronasal organ. Image published in: Kahl M et al. (2024) Copyright © 2024. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue calb2.L calretinin X. laevis Sometime during NF stage 48 to NF stage 52 olfactory sensory neuron olfactory epithelium Jacobson's organ s100z.L gm625, s100-zeta X. laevis Sometime during NF stage 48 to NF stage 52 olfactory sensory neuron olfactory epithelium Image source: Published Permanent Image Page Printer Friendly View XB-IMG-199039