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XB-PHENO-20915

Xla Wt + neurog3-GR + insm1 MO + DEX, Oropeza D and Horb M (2012)

Experiment + Assay: Xla Wt + neurog3-GR + insm1 MO + DEX + NF32 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(neurog3.S-GR) neurog3.S
neurog3.L
 NF stage 4 (8-cell) vegetal blastomere
morpholino injection insm1 MO1 insm1.L
insm1.S
 NF stage 4 (8-cell) vegetal blastomere
chemical dexamethasone
NF stage 12 whole organism
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 32  
Expression Phenotypes (3):
decreased amount ins expression in endoderm
decreased amount ins expression in foregut endoderm
decreased amount ins expression in pancreas
Source: Fig 6d
Article: XB-ART-44499, PubMed