Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-PHENO-21724

Xla Wt + Mmu.ascl1S62A,S88A,S185A,S189A,S202A,S218A + ccna2 + cdk2, Wylie LA et al. (2015)

Experiment + Assay: Xla Wt + Mmu.ascl1S62A,S88A,S185A,S189A,S202A,S218A + ccna2 + cdk2 + NF18-19 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection cdk2.L cdk2.S
cdk2.L
 NF stage 2 (2-cell) blastomere
mRNA injection ccna2.L ccna2.L
ccna2.S
 NF stage 2 (2-cell) blastomere
mRNA injection Tg(Mmu.ascl1S62A,S88A,S185A,S189A,S202A,S218A) ascl1.L
ascl1.S
 NF stage 2 (2-cell) blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 18 to NF stage 19  
Expression Phenotypes (3):
decreased amount hand2.L expression in anterior placodal area
increased amount phox2a.S expression in anterior placodal area
increased amount tubb2b.S expression in neural plate border
Source: Fig.6.a,b
Article: XB-ART-50246, PubMed