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XB-PHENO-21857

Xla Wt + hes1-GR + DEX + aphidicolin + hydroxyurea, Vega-López GA et al. (2015)

Experiment + Assay: Xla Wt + hes1-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(hes1-GR) hes1.L
hes1.S
 NF stage 4 (8-cell) blastomere
chemical dexamethasone
NF stage 11 whole organism
chemical hydroxyurea
NF stage 11 whole organism
chemical aphidicolin
NF stage 11 whole organism
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 16  
Expression Phenotypes (1):
decreased amount sox2.L expression in neural plate
Source: fig.7.g
Article: XB-ART-50752, PubMed