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XB-PHENO-22865

Xla Wt + pias4 + smad2 + animal cap explant, Daniels M et al. (2004)

Experiment + Assay: Xla Wt + pias4 + smad2 + animal cap explant + NF10.5 (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection smad2.L smad2.L
smad2.S
 NF stage 2 (2-cell) animal blastomere
mRNA injection pias4.L pias4.S
pias4.L
 NF stage 2 (2-cell) animal blastomere
explant  
NF stage 8 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 10.5  
Expression Phenotypes (1):
increased amount pias4.L expression in animal cap
Source: fig.3.D.a
Article: XB-ART-2861, PubMed