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XB-PHENO-22895

Xla Wt + ctnnb1tm1 + animal cap explant, Zorn AM et al. (1999)

Experiment + Assay: Xla Wt + ctnnb1tm1 + animal cap explant + gastrula stage (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(ctnnb1)tm1Rtmn ctnnb1.L
ctnnb1.S
 NF stage 3 (4-cell) animal blastomere
explant  
NF stage 9 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR gastrula stage  
Expression Phenotypes (2):
increased amount nodal3.1.L expression in animal cap
increased amount sia1.L expression in animal cap
Source: fig.3.f
Article: XB-ART-12045, PubMed