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XB-PHENO-22916

Xla Wt + VP16-ventx2.2-GR + CHX + DEX + animal cap explant, Rankin SA et al. (2011)

Experiment + Assay: Xla Wt + VP16-ventx2.2-GR + CHX + DEX + animal cap explant + NF11 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(VP16-ventx2.2-GR) ventx2.2.L
ventx2.2.S
 NF stage 5 (16-cell) ventral vegetal hemisphere blastomere
explant  
blastula stage animal cap
chemical dexamethasone
blastula stage animal cap
chemical cycloheximide
blastula stage animal cap
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 11  
Expression Phenotypes (3):
decreased amount chrd.1.L expression in animal cap
increased amount hhex.L expression in animal cap
increased amount otx2.L expression in animal cap
Source: fig.9.a
Article: XB-ART-42631, PubMed