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XB-PHENO-22994

Xla Wt + ctnnb1-GFP + sox17b.1 + animal cap explant, Sinner D et al. (2004)

Experiment + Assay: Xla Wt + ctnnb1-GFP + sox17b.1 + animal cap explant + gastrula stage (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection sox17b.1.L sox17b.1.L
sox17b.1.S
 NF stage 2 (2-cell) blastomere
mRNA injection Tg(CMV:ctnnb1-GFP) ctnnb1.S
ctnnb1.L
 NF stage 2 (2-cell) blastomere
explant  
NF stage 8 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR gastrula stage  
Expression Phenotypes (2):
increased amount foxa2.L expression in animal cap
increased amount hnf1b.L expression in animal cap
Source: fig.6
Article: XB-ART-3496, PubMed