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XB-PHENO-23011

Xla Wt + sox17b-GR + CHX + DEX + animal cap explant, Sinner D et al. (2004)

Experiment + Assay: Xla Wt + sox17b-GR + CHX + DEX + animal cap explant + NF11 (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(sox17b-GR) sox17b.1.L
sox17b.1.S
 NF stage 2 (2-cell) blastomere
explant  
NF stage 8 animal cap
chemical cycloheximide
NF stage 9 animal cap
chemical dexamethasone
NF stage 10 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 11  
Expression Phenotypes (6):
increased amount a2m.L expression in animal cap
increased amount foxa1.L expression in animal cap
increased amount foxa2.L expression in animal cap
increased amount hnf1b.L expression in animal cap
increased amount nodal.L expression in animal cap
increased amount sox17a.L expression in animal cap
Source: fig.2
Article: XB-ART-3496, PubMed