XB-PHENO-23027
Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO, Rankin SA et al. (2012)
| Experiment + Assay: |
Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO +
NF35/36 (in situ hybridization)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
morpholino injection
|
osr1 MO1
|
osr1
|
NF stage 4 (8-cell)
|
blastomere
|
|
morpholino injection
|
osr2 MO1
|
osr2
|
NF stage 4 (8-cell)
|
blastomere
|
|
mRNA injection
|
Tg(Mmu.osr1-GR)
|
osr1
|
NF stage 4 (8-cell)
|
blastomere D2
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
in situ hybridization
|
NF stage 35 and 36
|
|
|
|
|
Expression Phenotypes (4):
|
|
mislocalised
bmp4
expression in lateral plate mesoderm
|
|
decreased amount
nkx2-1
expression in lung bud
|
|
decreased amount
sox2
expression in foregut
|
|
decreased amount
wnt2b
expression in lung bud
|
|
