XB-PHENO-23207
Xla Wt + sia1-GR + CHX + DEX + animal cap explant, Rankin SA et al. (2011)
| Experiment + Assay: |
Xla Wt + sia1-GR + CHX + DEX + animal cap explant +
NF11 (in situ hybridization)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
mRNA injection
|
Tg(sia1.L-GR)
|
sia1.L
sia1.S
|
NF stage 5 (16-cell)
|
ventral vegetal hemisphere blastomere
|
|
explant
|
|
|
blastula stage
|
animal cap
|
|
chemical
|
dexamethasone
|
|
blastula stage
|
animal cap
|
|
chemical
|
cycloheximide
|
|
blastula stage
|
animal cap
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
in situ hybridization
|
NF stage 11
|
|
|
|
|
Expression Phenotypes (5):
|
|
increased amount
chrd.1.L
expression in animal cap
|
|
decreased amount
hhex.L
expression in animal cap
|
|
increased amount
lhx1.L
expression in animal cap
|
|
increased amount
otx2.L
expression in animal cap
|
|
increased amount
ventx1.2
expression in animal cap
|
|
