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XB-PHENO-24279

Xla Wt + esr1-GR + DEX + CHX, Tanaka K et al. (2014)

Experiment + Assay: Xla Wt + esr1-GR + DEX + CHX + NF25 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(esr1-GR) esr1 (Xla)
 NF stage 3 (4-cell)
chemical dexamethasone
NF stage 22 embryo
chemical cycloheximide
NF stage 22 embryo
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 25 to NF stage 25  
Expression Phenotypes (1):
absent pitx2.L expression in lateral plate mesoderm
Source: Fig. 2 B C
Article: XB-ART-48386, PubMed