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XB-PHENO-25713

Xtr Wt + cacna1c CRISPR + kcnh6 MO, Sempou E et al. (2022)

Experiment + Assay: Xtr Wt + cacna1c CRISPR + kcnh6 MO + NF10 (in situ hybridization)
Background: Xtr.NigerianKhok
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection kcnh6 MO1 kcnh6
 NF stage 1 whole organism
CRISPR cacna1c gRNA1 cacna1c
 NF stage 1 whole organism
CRISPR cacna1c gRNA1 cacna1c
 NF stage 1 whole organism
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 10  
Anatomical Phenotypes: abnormal blastopore morphology , abnormal gastrulation
Expression Phenotypes (1):
increased amount trim33 expression in animal cap
Source: Fig. 5a_c3
Article: XB-ART-59330, PubMed