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XB-PHENO-25834

Xla Wt + kmt5c MO + kmt5b MO (H1/H2 double KD), Angerilli A et al. (2023)

Experiment + Assay: Xla Wt + kmt5c MO + kmt5b MO (H1/H2 double KD) + NF28 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection kmt5b MO1 kmt5b (Xla)
 NF stage 2 (2-cell) animal pole
morpholino injection kmt5c MO1 kmt5c (Xla)
 NF stage 2 (2-cell) animal pole
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 28  
Anatomical Phenotypes: abnormal embryo morphology , abnormally decreased number of cilium in the ciliated cell , abnormal multiciliated cell morphology , decreased length of tail
Source: Fig. S1: B r2c2; D r3c2
Article: XB-ART-59747, PubMed