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XB-PHENO-26029

Xla Wt + pax8 + CHX + DEX + animal cap amputation, Buisson I et al. (2015)

Experiment + Assay: Xla Wt + pax8 + CHX + DEX + animal cap amputation + NF9-10 (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection pax8.L pax8.L
 NF stage 3 (4-cell) blastomere
chemical cycloheximide
NF stage 9
chemical dexamethasone
NF stage 9
amputation/regeneration  
NF stage 9 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 9 to NF stage 10  
Expression Phenotypes (1):
increased amount hnf1b.L expression in animal cap
Article: XB-ART-49971, PubMed