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XB-PHENO-27145

Xla Wt + ctnnb1 MO, Azbazdar Y and De Robertis EM (2024)

Experiment + Assay: Xla Wt + ctnnb1 MO + NF28 (in situ hybridization)
Background: Xla.B strain x J strain
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ctnnb1 MO1 ctnnb1
NF stage 3 (4-cell) left-ventral blastomere
morpholino injection ctnnb1 MO1 ctnnb1
NF stage 3 (4-cell) right-ventral blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 28  
Anatomical Phenotypes: wholly ventralized embryo
Expression Phenotypes (4):
absent sox2.L expression in brain
absent sox2.L expression in central nervous system
absent sox2.L expression in eye
absent sox2.L expression in neural tube
Source: Fig S3 B
Article: XB-ART-60778, PubMed