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XB-PHENO-27146

Xla Wt + ctnnb1 MO, Azbazdar Y and De Robertis EM (2024)

Experiment + Assay: Xla Wt + ctnnb1 MO + NF10.5 (in situ hybridization)
Background: Xla.B strain x J strain
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ctnnb1 MO1 ctnnb1
 NF stage 3 (4-cell) left-ventral blastomere
morpholino injection ctnnb1 MO1 ctnnb1
 NF stage 3 (4-cell) right-ventral blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 10.5  
Expression Phenotypes (1):
absent otx2.L expression in dorsal marginal zone
Source: Fig. S3 E
Article: XB-ART-60778, PubMed