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XB-PHENO-27203

Xla Wt + dorsal Tg(hhex:lambdaVP2), Brickman JM et al. (2000)

Experiment + Assay: Xla Wt + dorsal Tg(hhex:lambdaVP2) + NF35/36 (whole-mount microscopy)
Background: Xla.Cambridge
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
transgenesis Tg(hhex:lambdaVP2)
NF stage 3 (4-cell) left-dorsal blastomere
transgenesis Tg(hhex:lambdaVP2) hhex.L
 NF stage 3 (4-cell) right-dorsal blastomere
Conditions:
Assay Type Assay Stages Antibody
whole-mount microscopy NF stage 35 and 36  
Anatomical Phenotypes: abnormal development of anterior region , abnormal embryo morphology , abnormal gastrulation , abnormal head , fused eyes
Expression Phenotypes (2):
mislocalised chrd.L expression in embryo
mislocalised gsc.L expression in embryo
Source: Fig. 2 BCD, Table 1
Article: XB-ART-11037, PubMed