| Summary |
|
| Synonym: |
Mintbody, CMV-H3K9ac-EGFP, Xla.Tg(CMV:scFv-eGFP) |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Outbred
|
| Paternal Line: |
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| Maternal Line: |
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| Description: |
CMV promoter driving expression of of the epigenetic sensor, H3K9ac-mintbody (modification-specific intracellular antibody). Transgenic X. laevis ubiquitously expressing a fluorescent modification-specific intracellular antibody (Mintbody) that is able to track histone H3 lysine 9 acetylation (H3K9ac) in vivo through nuclear enhanced green fluorescent protein (EGFP) fluorescence. H3K9ac-Mintbody consists of a single-chain variable fragment (scFv) antibody against H3K9ac and enhanced Green Fluorescent Protein (eGFP). H3K9ac-Mintbody specifically binds to H3K9ac and consequently accumulates in nuclei. The dynamics of acetylation and active chromatin status can therefore be observed in vivo as intensity of nuclear EGFP fluorescence. |
| Phenotype Description: |
Ubiquitous expression of green fluorescent sensor of histone H3 lysine 9 acetylation. Robust fluorescence observed in the nuclei of somites, and in regenerating notochord. |
| Color Morph: |
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| Breeding Type: |
OUTBRED
|
| Lab of Origin: |
Ueno lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
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| MTA Required: |
No
|
| Public: |
Yes
|
| Catalogue Number: |
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