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XB-LINE-803
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Line Name: Xla.Tg(Xtr.ins:dnthra-eGFP)Brown
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| Summary | |
|---|---|
| Synonym: | pcs2::Insulin::TRDN-GFP3 |
| Species: | Xenopus laevis |
| Background Strain: | Xla.Outbred |
| Paternal Line: | |
| Maternal Line: | |
| Description: | 5.4 kb X. tropicalis insulin promoter driving dominant negative form of the thyroid receptor alpha ( (aka TRDN)) fused to GFP. The pInsulin-TRDN-GFPconstruct was made by amplifying the 5.4 kb fragment of the promoter by PCR, using primers that incorporated a Xho1 site at the 5' end and Sma1 at the 3' end, and this PCR-product was ligated into the pCS2-CMV-TRDN-flex-GFP that had been cleaved by Sal1 (at 5'and HindIII (at 3') replacing the existing CMV promoter sequence. |
| Phenotype Description: | GFP in pancreatic Beta-cells. |
| Color Morph: | |
| Breeding Type: | UNKNOWN |
| Lab of Origin: | Brown Lab |
| Line Type: | Transgenic |
| Mutated Gene(s): | |
| MTA Required: | Yes |
| Public: | Yes |
| Catalogue Number: |
| Stock Center | RRID | Generation | Availability | Mutant Details |
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| No availability, contact the Lab of Origin for information about this Xenopus line or strain. Articles using these frogs are listed on the Attribution Tab. | ||||
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