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XB-LINE-920
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Line Name: Xla.Tg(kdr:eGFP)Pec
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| Summary | |
|---|---|
| Synonym: | Xl.Tg(flk1:eGFP) |
| Species: | Xenopus laevis |
| Background Strain: | Xla.Outbred |
| Paternal Line: | |
| Maternal Line: | |
| Description: | The Xenopus laevis Flk1 (VEGFR-2) promoter and first intron were used to drive GFP. See Figure 1 and Figure 2 in Ny et. Al. Biol Open. 2013 Jul 11; 2(9):882-90. A genomic fragment containing 2.5 kb of promoter sequence up to exon 2, including the complete intron 1 containing enhancer elements are essential for the correct expression pattern, was isolated and cloned into pBSK(+) Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the xlFlk1 promoter. REMI method. |
| Phenotype Description: | GFP expression in blood and lymphatic vessels. Earliest specific GFP signal visible is at NF stage 35-36 (in posterior cardinal vein and intersomitic vessels). GFP signal in all blood vessels and lymphatic vessels from NF stage 42 onwards. |
| Color Morph: | albino |
| Breeding Type: | UNKNOWN |
| Lab of Origin: | Carmeliet Lab |
| Line Type: | Transgenic |
| Mutated Gene(s): | |
| MTA Required: | No |
| Public: | Yes |
| Catalogue Number: | waiting for EXRC RRID |
| Stock Center | RRID | Generation | Availability | Mutant Details | Information  |
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| NXR (US) | |||||
| EXRC (Europe) | |||||
| NBRP (Japan) | |||||
| XLRRI (US) | |||||
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