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Figure 2 . Lateral view of a transgenic tadpole [Xla.Tg(rho:Eco.nfsA)] (stage 51) under bright field illumination (a), blue light (b) or both (c, the tungsten lamp was dimmed compared to a). The bright green fluorescence from the transgene is seen in the eye. d: Dorsal view of a transgenic tadpole (stage 48) under bright field illumination. The position of the eyes (e), optic nerve (on), olfactory organ (o), cerebral hemispheres (c) and pineal (p) are indicated. A higher magnification image of the pineal under bright field (e) and blue light (f). GFPâspecific fluorescence is seen as a punctate pattern in pineal. g: The anterior portion of a transgenic juvenile frog illuminated with both tungsten lamp and blue light.
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Figure 4 . Confocal images of a retina from a transgenic tadpole [Xla.Tg(rho:Eco.nfsA)Zuber] produced using pXOP(â5500/+41)GFP. Two separate sections are shown (A and B, C and D). The images under bright field (A, C) were obtained with both bright field and blue light, so the GFP fluorescence (green) is visible in these images. Sections were also viewed for fluorescence from GFP (green) and Cy3 which revealed B630N rod opsin staining (blue) (B, D). Cones are indicated, as well as opsinâ and GFPâpositive rods.
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