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Map of the heat-shock-inducible Dkk1GFP transgene with lens labeling. The bright RFP variant, tdTomato under control of the 2.2-kb óâÂÂcrystallin promoter, was introduced downstream of hsp70-Dkk1GFP.
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Map of the heat-shock-inducible Dkk1GFP transgene with lens labeling. The bright RFP variant, tdTomato under control of the 2.2-kb γ–crystallin promoter, was introduced downstream of hsp70-Dkk1GFP.
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hsDkk1 inhibited the forelimb bud regeneration in tadpoles. (A and B) Dorsal view of the left forelimb bud at stage 54. A paddle-shaped forelimb bud can be seen in a cavity dorsal to the posterior portion of the gill region (A). The hsDkk1GFP expression was induced in the entire tadpole body, including the forelimb bud region, by heat-shock (B).
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