|
XB-LINE-1354
The following transgenes are currently part of this line:
Description: X. tropicalis HAL2 promoter region was PCR amplified- the product was used for the nested PCR with primers bearing a KpnI site and an AgeI site, digested with KpnI and AgeI, and cloned into AcGFP1.1 vector (Clontech). A 4.4-kb HAL2 promoter in AcGFP1.1 was subcloned into the KpnI-AgeI digested double-promoter construct pDPCG-SceI -to produce a transgenic construct with the HAL2 promoter driving eGFP expression and cryga in lens,which allows easy identification of transgenic tadpoles
|
||||||||||
Insertion Sites:
|
||||||||||