Xenbase Image ID: 130034

Fig. 1. Expression of x-Dll3, x-Nkx2.1, and x-Lhx7 in embryonic Xenopus forebrain. The x-Nkx2.1 protein and GABA neurotransmitter are visualized by immunofluorescence (IF), and the x-Lhx7 and x-Dll3 transcripts are detected by in situ hybridization (ISH, red) revealed by Fast Red and observed in fluorescence (x-Lhx7) or in bright field microscopy (x-Dll3). Panels A–C show lateral views of in toto dissected brains stained with the indicated gene marker at stage 35. Anterior is left, dorsal is up, and the shape of the brain is outlined by a dotted line for fluorescence pictures. (D) Optical and confocal sections through the basal telencephalon of stage 35 embryos labeled for x-Dll3 mRNA or double-labeled for x-Nkx2.1 protein and x-Lhx7 mRNA. Dotted lines delineate subdivisions and neuroepithelial layers. Hyp, hypothalamus; mge, lge, medial and lateral ganglionic eminence; pa, pallium; spa, subpallium; vz/svz/mz, ventricular, subventricular, and marginal zone. Image published in: Bachy I and Rétaux S (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 35 and 36 medial ganglionic eminence hypothalamus diencephalon lhx8.L lhx7, x-Lhx7 X. laevis Throughout NF stage 35 and 36 medial ganglionic eminence diencephalon subpallium zona limitans intrathalamica forebrain brain dlx5.L dll3, X-dll3 X. laevis Throughout NF stage 35 and 36 diencephalon subpallium prethalamus lateral ganglionic eminence medial ganglionic eminence zona limitans intrathalamica Image source: Published Larger Image Printer Friendly View

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