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Fig. 5. Distribution of cH2AX in ZZ and ZW gonads during development. Immunostaining with anti-Dmrt1 and anticH2AX antibodies was performed on gonadal sections from ZW and ZZ tadpoles at stages 53 and 62 (A) and on gonadal sections from a ZZ adult frog (B). Nuclei were stained with TO-PRO. Oc, oocyte; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte. White arrowheads and arrows indicate Dmrt1- or cH2AX-positive primary GSCs and leptotene/zygotene oocytes, respectively. Image published in: Fujitani K et al. (2016) © 2016 The Authors. Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue dmrt1.L dmrt1-a, dmrt1-b, dmt1, xdmrt1 X. laevis Throughout adult frog stage secondary spermatogonium primary spermatogonium testis h2ax.S h2afxl, h2a.x, h2a/x, h2axl X. laevis Throughout adult frog stage secondary spermatogonium primary spermatogonium testis spermatocyte dmrt1.L dmrt1-a, dmrt1-b, dmt1, xdmrt1 X. laevis Throughout NF stage 53 ovary primordial germ cell testis h2ax.S h2afxl, h2a.x, h2a/x, h2axl X. laevis Throughout NF stage 54 to prometamorphosis stage testis ovary primordial germ cell dmrt1.L dmrt1-a, dmrt1-b, dmt1, xdmrt1 X. laevis Throughout NF stage 62 secondary oogonium primary oogonium primary spermatogonium ovary h2ax.S h2afxl, h2a.x, h2a/x, h2axl X. laevis Throughout NF stage 62 oocyte testis ovary Image source: Published Larger Image Printer Friendly View

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