Xenbase Image ID: 158182

Fig. 3. WISH of vegetal pole transcripts. The expression of a subset of vegetally enriched mRNAs was analyzed during oogenesis and embryo development by WISH. Expression patterns are grouped according to mRNA pathway: early (A), late (B) or both (C). xpat expression marks germ plasm. Probes, developmental stages and developmental structures are indicated in the illustrations at the top: germ plasm/PGCs (purple), pronephros (pink), ventral blood islands (lime green), eye (black), lens (white), otic vesicle (gray), cranial ganglia (yellow), brachial arches (green), nasal placodes (teal), intersegmental region (brown), notochord (orange), brain and neural tube (blue). Transcripts detected in PGCs at the tailbud stage are shown at higher magnification in insets. WISH analysis was performed on ≥40 total embryos from at least two adult female frogs. Dorsal (D), ventral (V), left (L), right (R), posterior (P), anterior (A), vegetal view (v); MC, mitochondrial cloud; N, nucleus; Oo, oocyte. Scale bars: 100†μm for stage I oocyte; 200†μm in remaining panels. Image published in: Owens DA et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sybu.L golsyn, ocsyn, snphl, syntabulin X. laevis Throughout NF stage 11 animal hemisphere pgat.L Xpat X. laevis Throughout NF stage 16 germ cell primordial germ cell efnb1.L cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 X. laevis Throughout NF stage 16 mesoderm pre-chordal neural plate mov10.L X. laevis Throughout NF stage 16 primordial germ cell otx1.L xotx1 X. laevis Throughout NF stage 16 primordial germ cell spire1.L LOC108719187, pEg6, spire X. laevis Throughout NF stage 16 primordial germ cell wnk2.L LOC108704008, LOC108704406, prkwnk2, sdccag43 X. laevis Throughout NF stage 16 primordial germ cell e2f1.L xE2F X. laevis Throughout NF stage 16 primordial germ cell sybu.L golsyn, ocsyn, snphl, syntabulin X. laevis Throughout NF stage 16 primordial germ cell neural plate pgat.L Xpat X. laevis Throughout NF stage 32 germ cell primordial germ cell efnb1.L cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 X. laevis Throughout NF stage 32 olfactory bulb duct of Cuvier aortic arch rras2.L LOC779088, tc21 X. laevis Throughout NF stage 32 spinal cord somite mov10.L X. laevis Throughout NF stage 32 germ cell primordial germ cell somite otx1.L xotx1 X. laevis Throughout NF stage 32 germ cell primordial germ cell eye sox7.L xSox7 X. laevis Throughout NF stage 32 pharynx mouth primordium spire1.L LOC108719187, pEg6, spire X. laevis Throughout NF stage 32 germ cell primordial germ cell wnk2.L LOC108704008, LOC108704406, prkwnk2, sdccag43 X. laevis Throughout NF stage 32 germ cell primordial germ cell spinal cord e2f1.L xE2F X. laevis Throughout NF stage 32 germ cell primordial germ cell sybu.L golsyn, ocsyn, snphl, syntabulin X. laevis Throughout NF stage 32 germ cell primordial germ cell spinal cord trigeminal nerve olfactory bulb pgat.L Xpat X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere efnb1.L cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere rras2.L LOC779088, tc21 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere mov10.L X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere otx1.L xotx1 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere sox7.L xSox7 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere spire1.L LOC108719187, pEg6, spire X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere wnk2.L LOC108704008, LOC108704406, prkwnk2, sdccag43 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere e2f1.L xE2F X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere sybu.L golsyn, ocsyn, snphl, syntabulin X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere pgat.L Xpat X. laevis Throughout oocyte stage I vegetal pole mitochondrial cloud efnb1.L cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 X. laevis Throughout oocyte stage I vegetal pole mitochondrial cloud rras2.L LOC779088, tc21 X. laevis Throughout oocyte stage I vegetal pole mitochondrial cloud e2f1.L xE2F X. laevis Throughout oocyte stage I vegetal pole mitochondrial cloud sybu.L golsyn, ocsyn, snphl, syntabulin X. laevis Sometime during oocyte stage I to oocyte stage VI vegetal pole mitochondrial cloud Image source: Published Larger Image Printer Friendly View

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