Fig. 3. WISH of vegetal pole transcripts. The expression of a subset of vegetally enriched mRNAs was analyzed during oogenesis and embryo development by WISH. Expression patterns are grouped according to mRNA pathway: early (A), late (B) or both (C). xpat expression marks germ plasm. Probes, developmental stages and developmental structures are indicated in the illustrations at the top: germ plasm/PGCs (purple), pronephros (pink), ventral blood islands (lime green), eye (black), lens (white), otic vesicle (gray), cranial ganglia (yellow), brachial arches (green), nasal placodes (teal), intersegmental region (brown), notochord (orange), brain and neural tube (blue). Transcripts detected in PGCs at the tailbud stage are shown at higher magnification in insets. WISH analysis was performed on â¥40 total embryos from at least two adult female frogs. Dorsal (D), ventral (V), left (L), right (R), posterior (P), anterior (A), vegetal view (v); MC, mitochondrial cloud; N, nucleus; Oo, oocyte. Scale bars: 100â μm for stage I oocyte; 200â μm in remaining panels.
Image published in: Owens DA et al. (2017)
Copyright © 2017. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| sybu.L | golsyn, ocsyn, snphl, syntabulin | X. laevis | Throughout NF stage 11 | animal hemisphere |
| pgat.L | Xpat | X. laevis | Throughout NF stage 16 | germ cell primordial germ cell |
| efnb1.L | cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 | X. laevis | Throughout NF stage 16 | mesoderm pre-chordal neural plate |
| mov10.L | X. laevis | Throughout NF stage 16 | primordial germ cell | |
| otx1.L | xotx1 | X. laevis | Throughout NF stage 16 | primordial germ cell |
| spire1.L | LOC108719187, pEg6, spire | X. laevis | Throughout NF stage 16 | primordial germ cell |
| wnk2.L | LOC108704008, LOC108704406, prkwnk2, sdccag43 | X. laevis | Throughout NF stage 16 | primordial germ cell |
| e2f1.L | xE2F | X. laevis | Throughout NF stage 16 | primordial germ cell |
| sybu.L | golsyn, ocsyn, snphl, syntabulin | X. laevis | Throughout NF stage 16 | primordial germ cell neural plate |
| pgat.L | Xpat | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell |
| efnb1.L | cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 | X. laevis | Throughout NF stage 32 | olfactory bulb duct of Cuvier aortic arch |
| rras2.L | LOC779088, tc21 | X. laevis | Throughout NF stage 32 | spinal cord somite |
| mov10.L | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell somite | |
| otx1.L | xotx1 | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell eye |
| sox7.L | xSox7 | X. laevis | Throughout NF stage 32 | pharynx mouth primordium |
| spire1.L | LOC108719187, pEg6, spire | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell |
| wnk2.L | LOC108704008, LOC108704406, prkwnk2, sdccag43 | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell spinal cord |
| e2f1.L | xE2F | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell |
| sybu.L | golsyn, ocsyn, snphl, syntabulin | X. laevis | Throughout NF stage 32 | germ cell primordial germ cell spinal cord trigeminal nerve olfactory bulb |
| pgat.L | Xpat | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| efnb1.L | cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| rras2.L | LOC779088, tc21 | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| mov10.L | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere | |
| otx1.L | xotx1 | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| sox7.L | xSox7 | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| spire1.L | LOC108719187, pEg6, spire | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| wnk2.L | LOC108704008, LOC108704406, prkwnk2, sdccag43 | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| e2f1.L | xE2F | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| sybu.L | golsyn, ocsyn, snphl, syntabulin | X. laevis | Throughout NF stage 6 (32-cell) | vegetal blastomere |
| pgat.L | Xpat | X. laevis | Throughout oocyte stage I | vegetal pole mitochondrial cloud |
| efnb1.L | cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 | X. laevis | Throughout oocyte stage I | vegetal pole mitochondrial cloud |
| rras2.L | LOC779088, tc21 | X. laevis | Throughout oocyte stage I | vegetal pole mitochondrial cloud |
| e2f1.L | xE2F | X. laevis | Throughout oocyte stage I | vegetal pole mitochondrial cloud |
| sybu.L | golsyn, ocsyn, snphl, syntabulin | X. laevis | Sometime during oocyte stage I to oocyte stage VI | vegetal pole mitochondrial cloud |
Image source: Published
Permanent Image Page
Printer Friendly View
XB-IMG-158182