Xenbase Image ID: 175264

Fig. 4. Spatial gene expression pattern of trio. Trio gene expression was analyzed by whole mount in situ hybridization. Embryonic stages are indicated. The detailed expression pattern is described in the text. A Embryo at cleavage stage, lateral view. B Blastula stage embryo, lateral view. C Gastrula stage embryo, blastopore lip is indicated. D Transverse section of embryo in C; section plane is indicated in C. E Control in situ hybridization using a sense trio probe; gastrula stage embryo. F,G Neurula stage embryos, dorsal view. H Tailbud stage embryo, dorsal view. I,J Transverse sections of embryo shown in H. Section planes are indicated in H. Arrowhead in I marks staining between the meso- and endoderm likely representing migrating NC cells. K-M Tailbud stage embryos, lateral view. Sense control at stage 28 is indicated in the insert in L. N Higher magnification of the head of the embryo shown in L. O Double in situ hybridization showing trio (blue) and twist (red) co-expression in cranial NC cells. P, Q Transverse sections of tadpole embryos analyzed by trio/twist double in situ hybridization. Section planes are indicated in O. Abbreviations: a: animal, b: brain; ba: branchial arches; bl: blastopore lip; cg: cement gland; e: eye; ec: ectoderm; ha: hyoidal arch; ma: mandibular arch; me: mesoderm; nc: neural crest; no: notochord; np: neural plate; npb: neural plate border; nt: neural tube; ov: otic vesicle; s: somites; pom: periocular mesenchyme; sc: sclerotome; tg: trigeminal ganglion; v: vegetal. Scale bar: 500 μm (I,J: 200 μm). Image published in: Kratzer MC et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue trio.L arhgef23, tgat X. laevis Throughout NF stage 10.5 ectoderm involuted dorsal mesoderm marginal zone trio.L arhgef23, tgat X. laevis Throughout NF stage 13 neural plate trio.L arhgef23, tgat X. laevis Throughout NF stage 16 neural plate border neural plate trio.L arhgef23, tgat X. laevis Throughout NF stage 22 neural plate somite sclerotome cranial neural crest neural tube notochord trio.L arhgef23, tgat X. laevis Throughout NF stage 23 cement gland primordium trigeminal placode neural tube somite head migratory neural crest cell trio.L arhgef23, tgat X. laevis Throughout NF stage 28 branchial arch hyoid arch mandibular arch brain eye otic vesicle trigeminal nerve neural tube somite trio.L arhgef23, tgat X. laevis Throughout NF stage 37 and 38 branchial arch cranial neural crest mandibular arch hyoid arch brain trio.L arhgef23, tgat X. laevis Throughout NF stage 6 (32-cell) animal hemisphere trio.L arhgef23, tgat X. laevis Throughout NF stage 9 animal hemisphere Image source: Published Larger Image Printer Friendly View

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