Fig. 4. Spatial gene expression pattern of trio. Trio gene expression was analyzed by whole mount in situ hybridization. Embryonic stages are indicated. The detailed expression pattern is described in the text. A Embryo at cleavage stage, lateral view. B Blastula stage embryo, lateral view. C Gastrula stage embryo, blastopore lip is indicated. D Transverse section of embryo in C; section plane is indicated in C. E Control in situ hybridization using a sense trio probe; gastrula stage embryo. F,G Neurula stage embryos, dorsal view. H Tailbud stage embryo, dorsal view. I,J Transverse sections of embryo shown in H. Section planes are indicated in H. Arrowhead in I marks staining between the meso- and endoderm likely representing migrating NC cells. K-M Tailbud stage embryos, lateral view. Sense control at stage 28 is indicated in the insert in L. N Higher magnification of the head of the embryo shown in L. O Double in situ hybridization showing trio (blue) and twist (red) co-expression in cranial NC cells. P, Q Transverse sections of tadpole embryos analyzed by trio/twist double in situ hybridization. Section planes are indicated in O. Abbreviations: a: animal, b: brain; ba: branchial arches; bl: blastopore lip; cg: cement gland; e: eye; ec: ectoderm; ha: hyoidal arch; ma: mandibular arch; me: mesoderm; nc: neural crest; no: notochord; np: neural plate; npb: neural plate border; nt: neural tube; ov: otic vesicle; s: somites; pom: periocular mesenchyme; sc: sclerotome; tg: trigeminal ganglion; v: vegetal. Scale bar: 500â¯Î¼m (I,J: 200â¯Î¼m).
Image published in: Kratzer MC et al. (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 10.5 | ectoderm involuted dorsal mesoderm marginal zone |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 13 | neural plate |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 16 | neural plate border neural plate |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 22 | neural plate somite sclerotome cranial neural crest neural tube notochord |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 23 | cement gland primordium trigeminal placode neural tube somite head migratory neural crest cell |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 28 | branchial arch hyoid arch mandibular arch brain eye otic vesicle trigeminal nerve neural tube somite |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 37 and 38 | branchial arch cranial neural crest mandibular arch hyoid arch brain |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 6 (32-cell) | animal hemisphere |
| trio.L | arhgef23, tgat | X. laevis | Throughout NF stage 9 | animal hemisphere |
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