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Figure 3. Whole mount in situ hybridization of up-regulated genes, grouped according to function as wound healing, inflammation response, and cell proliferation genes. Tailbud stage embryo (A, D, G, J, L, N), regenerating tail on day 2 (B, E, H), and regenerating tail on day 5 (C, F, I, K, M, O) were hybridized with DIG-labeled RNA probes. Lateral view, dorsal side up, anterior to the left. White arrowheads, notochord; white arrows, blood cells; red arrowhead, uncharacterized cells; black arrowheads, wound epidermis; broken line, the plane of amputation. AâC: Expression of annexin II (XL073e04). DâF: Expression of PAI1 (XL074b06). GâI: Expression of cathepsin B (XL040b13). J, K: Expression of complement component C3 (XL028a02). L, M: Expression of histone H3.3B (XL092g11). N, O: Expression of serotransferrin precursor (XL040d11).