Figure 3. Whole mount in situ hybridization of up-regulated genes, grouped according to function as wound healing, inflammation response, and cell proliferation genes. Tailbud stage embryo (A, D, G, J, L, N), regenerating tail on day 2 (B, E, H), and regenerating tail on day 5 (C, F, I, K, M, O) were hybridized with DIG-labeled RNA probes. Lateral view, dorsal side up, anterior to the left. White arrowheads, notochord; white arrows, blood cells; red arrowhead, uncharacterized cells; black arrowheads, wound epidermis; broken line, the plane of amputation. AâC: Expression of annexin II (XL073e04). DâF: Expression of PAI1 (XL074b06). GâI: Expression of cathepsin B (XL040b13). J, K: Expression of complement component C3 (XL028a02). L, M: Expression of histone H3.3B (XL092g11). N, O: Expression of serotransferrin precursor (XL040d11).
Image published in: Tazaki A et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| anxa2.L | annexin a2, annexin II, anx2, anx2l4, anxa2-a, anxa2-b, cal1h, lip2, Lipocortin II, lpc2, lpc2d, p36, pap-i | X. laevis | Throughout NF stage 28 | otic vesicle notochord endoderm cement gland midbrain pineal gland trigeminal ganglion |
| serpine1.L | PAI 1 | X. laevis | Throughout NF stage 28 | notochord axial mesoderm |
| ctsb.S | apps, cathepsin B, cpsb | X. laevis | Throughout NF stage 28 | otic vesicle eye pharyngeal arch mandibular arch hyoid arch branchial arch pronephric mesenchyme head region proctodeum pharyngeal region foregut endoderm |
| h3-5.L | h3f3c, LOC108716488 | X. laevis | Throughout NF stage 28 | otic vesicle eye hindbrain spinal cord pharyngeal arch head region chordoneural hinge brain tail tip tail bud mandibular arch hyoid arch branchial arch pronephric mesenchyme |
| c3.L | asp, ccc3, cpamd1, LOC108703407 | X. laevis | Throughout NF stage 28 | otic vesicle endoderm hindbrain pharyngeal arch mandibular arch hyoid arch branchial arch dorsal tail fin tail fin cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest migratory neural crest cell periocular region cephalic nerve |
| tf.L | lactoferrin, lactotransferrin, ltf, serotransferin precursor, tf-a, tf-b, transferrin | X. laevis | Throughout NF stage 28 | endoderm pharyngeal arch chordoneural hinge foregut proctodeum |
| anxa2.L | annexin a2, annexin II, anx2, anx2l4, anxa2-a, anxa2-b, cal1h, lip2, Lipocortin II, lpc2, lpc2d, p36, pap-i | X. laevis | Throughout NF stage 32 | regenerating tail |
| serpine1.L | PAI 1 | X. laevis | Throughout NF stage 32 | notochord regenerating tail |
| ctsb.S | apps, cathepsin B, cpsb | X. laevis | Throughout NF stage 32 | regenerating tail |
| anxa2.L | annexin a2, annexin II, anx2, anx2l4, anxa2-a, anxa2-b, cal1h, lip2, Lipocortin II, lpc2, lpc2d, p36, pap-i | X. laevis | Throughout NF stage 33 and 34 | blood regenerating tail |
| serpine1.L | PAI 1 | X. laevis | Throughout NF stage 33 and 34 | notochord chordoneural hinge regenerating tail |
| ctsb.S | apps, cathepsin B, cpsb | X. laevis | Throughout NF stage 33 and 34 | blood regenerating tail |
| h3-5.L | h3f3c, LOC108716488 | X. laevis | Throughout NF stage 33 and 34 | regenerating tail tail tip |
| c3.L | asp, ccc3, cpamd1, LOC108703407 | X. laevis | Throughout NF stage 33 and 34 | blood endoderm dorsal fin regenerating tail wound epidermis |
| tf.L | lactoferrin, lactotransferrin, ltf, serotransferin precursor, tf-a, tf-b, transferrin | X. laevis | Throughout NF stage 33 and 34 | regenerating tail tail tip |
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