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Fig. 2. Osr1/Osr2 are required for respiratory specification and tracheal-esophageal separation. (A-L′) Embryos were co-injected with control MM-MOs or Osr1/Osr2-MOs and GR-mOsr1 RNA (250 pg) in both dorsal-vegetal blastomeres at the eight-cell stage (bilateral injection) and then cultured ±dexamethasone from stage 20 onwards. (A-C,G-I) Analysis by in situ hybridization of nkx2.1 at stage 34/35 (A-C) and sftpc at stage 42 (G-I). (D-F′,J-L′) Confocal immunofluorescence of Nkx2.1 (green) and Sox2 (red) at stage 35/36 (D-F) and stage 42 (J-L). es, esophagus; tr, trachea; lb, lung buds; st, stomach, Scale bars: 100 μm. [Curators Note: all embryos X. laevis] Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lung endoderm respiratory system lung primordium nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lung endoderm foregut thyroid primordium brain forebrain olfactory bulb sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 33 and 34 to NF stage 42 endoderm esophagus stomach nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 42 lung endoderm trachea foregut lung primordium sftpc.S SP-C, SPC, surfc, xSP-C X. laevis Throughout NF stage 42 lung endoderm respiratory system Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.2.b Expression Phenotype decreased amount nkx2-1 expression in foregut epithelium Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.2.b,e,e^1 Anatomical Phenotype absent lung bud Expression Phenotype decreased amount nkx2-1 expression in lung bud Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.2.e,e^1 Anatomical Phenotype abnormal development of foregut abnormal development of lung Expression Phenotype decreased amount nkx2-1 expression in foregut epithelium decreased amount sox2 expression in foregut epithelium Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.2.h Expression Phenotype decreased amount sftpc expression in lung bud Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.2.h,k,k^1 Anatomical Phenotype absent lung bud Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.2.k Expression Phenotype decreased amount nkx2-1 expression in pulmonary epithelium decreased amount nkx2-1 expression in tracheal epithelium decreased amount sox2 expression in esophageal epithelium Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.2.k^1 Anatomical Phenotype decreased size of the stomach Expression Phenotype decreased amount sox2 expression in stomach epithelium Larger Image Printer Friendly View

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