Xenbase Image ID: 86931

Fig. 2. Whole-mount in situ hybridization illustrating the expression of PACAP (A–I) and PAC1 (J–O) genes in embryos at different developing stages. A–I show PACAP mRNA distribution in embryos at stage 18, 21, 23, 25, 26, 27, 29, 31, and 33, respectively. PACAP mRNA signals were first localized at stage 21 in the embryonic spinal cord (small arrow in B). At the later stages, PACAP mRNA signals were continuously detected in the dorsal part of the entire spinal cord and middle/posterior rhombencephalon (small arrows in B–I). Hybridization signals were not present in the embryonic forebrain over the course of study (open arrows in D–H). Starting from stage 23 (C), however, PACAP gene transcripts were observed in the trigeminal ganglia (large arrows in C–H). J–O show PAC1 mRNA distribution in embryos at stage 18, 21, 26, 28, 31, and 33, respectively. PAC1 mRNA signals were first detected in the forebrain area at stage 21 (solid arrow in K). At the later stages, PAC1 mRNA signals were continuously detected in deep portions of the forebrain, including the tuberculum posterior, prosencephalon, mesencephalon, rhombencephalon, and anterior spinal cord (solid arrows in L–O). PAC1 gene transcripts were not discernible in the forebrain of embryos at stages 18 (open arrow in J) or in the middle/posterior spinal cord at any stage (open arrows in K–O). SC, spinal cord; F, forebrain; TG, trigeminal ganglion. Scale bar 5 2 mm. Image published in: Hu Z et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue adcyap1.L pacap X. laevis Throughout NF stage 21 neural tube posterior neural tube adcyap1r1.L pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R X. laevis Throughout NF stage 21 forebrain anterior neural tube adcyap1.L pacap X. laevis Sometime during NF stage 23 to NF stage 31 spinal cord trigeminal ganglion adcyap1r1.L pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R X. laevis Sometime during NF stage 26 to NF stage 28 brain adcyap1r1.L pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R X. laevis Sometime during NF stage 26 to NF stage 28 brain adcyap1r1.L pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R X. laevis Sometime during NF stage 31 to NF stage 33 and 34 spinal cord brain forebrain hindbrain diencephalon midbrain adcyap1.L pacap X. laevis Throughout NF stage 33 and 34 spinal cord Image source: Published Larger Image Printer Friendly View

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