Fig. 2. Whole-mount in situ hybridization illustrating the expression of PACAP (AâI) and PAC1 (JâO) genes in embryos at different developing stages. AâI show PACAP mRNA distribution in embryos at stage 18, 21, 23, 25, 26, 27, 29, 31, and 33, respectively. PACAP mRNA signals were first localized at stage 21 in the embryonic spinal cord (small arrow in B). At the later stages, PACAP mRNA signals were continuously detected in the dorsal part of the entire spinal cord and middle/posterior rhombencephalon (small arrows in BâI). Hybridization signals were not present in the embryonic forebrain over the course of study (open arrows in DâH). Starting from stage 23 (C), however, PACAP gene transcripts were observed in the trigeminal ganglia (large arrows in CâH). JâO show PAC1 mRNA distribution in embryos at stage 18, 21, 26, 28, 31, and 33, respectively. PAC1 mRNA signals were first detected in the forebrain area at stage 21 (solid arrow in K). At the later stages, PAC1 mRNA signals were continuously detected in deep portions of the forebrain, including the tuberculum posterior, prosencephalon, mesencephalon, rhombencephalon, and anterior spinal cord (solid arrows in LâO). PAC1 gene transcripts were not discernible in the forebrain of embryos at stages 18 (open arrow in J) or in the middle/posterior spinal cord at any stage (open arrows in KâO). SC, spinal cord; F, forebrain; TG, trigeminal ganglion. Scale bar 5 2 mm.
Image published in: Hu Z et al. (2001)
Copyright © 2001. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| adcyap1.L | pacap | X. laevis | Throughout NF stage 21 | neural tube posterior neural tube |
| adcyap1r1.L | pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R | X. laevis | Throughout NF stage 21 | forebrain anterior neural tube |
| adcyap1.L | pacap | X. laevis | Sometime during NF stage 23 to NF stage 31 | spinal cord trigeminal ganglion |
| adcyap1r1.L | pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R | X. laevis | Sometime during NF stage 26 to NF stage 28 | brain |
| adcyap1r1.L | pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R | X. laevis | Sometime during NF stage 26 to NF stage 28 | brain |
| adcyap1r1.L | pac1, pacapr, pacapri, PACAP-type-I-receptor, P-I-R | X. laevis | Sometime during NF stage 31 to NF stage 33 and 34 | spinal cord brain forebrain hindbrain diencephalon midbrain |
| adcyap1.L | pacap | X. laevis | Throughout NF stage 33 and 34 | spinal cord |
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