Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Rep
2014 Feb 27;64:737-47. doi: 10.1016/j.celrep.2014.01.024.
Show Gene links
Show Anatomy links
Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus.
Shen W
,
Liu HH
,
Schiapparelli L
,
McClatchy D
,
He HY
,
Yates JR
,
Cline HT
.
???displayArticle.abstract???
Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine analog azidohomoalanine (AHA) combined with the multidimensional protein identification technique (MudPIT) to identify proteins that are synthesized in the tadpolebrain over 24 hr. We induced conditioning-dependent plasticity of visual avoidance behavior, which required N-methyl-D-aspartate (NMDA) and Ca(2+)-permeable α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, αCaMKII, and rapid protein synthesis. Combining BONCAT with western blots revealed that proteins including αCaMKII, MEK1, CPEB, and GAD65 are synthesized during conditioning. Acute synthesis of CPEB during conditioning is required for behavioral plasticity as well as conditioning-induced synaptic and structural plasticity in the tectal circuit. We outline a signaling pathway that regulates protein-synthesis-dependent behavioral plasticity in intact animals, identify newly synthesized proteins induced by visual experience, and demonstrate a requirement for acute synthesis of CPEB in plasticity.
AGRANOFF,
PUROMYCIN EFFECT ON MEMORY FIXATION IN THE GOLDFISH.
1964, Pubmed
AGRANOFF,
PUROMYCIN EFFECT ON MEMORY FIXATION IN THE GOLDFISH.
1964,
Pubmed
Aizenman,
Visually driven modulation of glutamatergic synaptic transmission is mediated by the regulation of intracellular polyamines.
2002,
Pubmed
,
Xenbase
Atkins,
Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II.
2004,
Pubmed
Beatty,
Two-color labeling of temporally defined protein populations in mammalian cells.
2008,
Pubmed
Berger-Sweeney,
Reduced extinction of hippocampal-dependent memories in CPEB knockout mice.
2006,
Pubmed
Best,
Click chemistry and bioorthogonal reactions: unprecedented selectivity in the labeling of biological molecules.
2009,
Pubmed
Bestman,
The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo.
2008,
Pubmed
,
Xenbase
Bhatia,
Software tool for researching annotations of proteins: open-source protein annotation software with data visualization.
2009,
Pubmed
Bowie,
Inward rectification of both AMPA and kainate subtype glutamate receptors generated by polyamine-mediated ion channel block.
1995,
Pubmed
Chen,
Visualizing long-term memory formation in two neurons of the Drosophila brain.
2012,
Pubmed
Chen,
The transcription factor MEF2 directs developmental visually driven functional and structural metaplasticity.
2012,
Pubmed
,
Xenbase
Choi,
Defining TNF-α and IL-1β induced nascent proteins: combining bio-orthogonal non-canonical amino acid tagging and proteomics.
2012,
Pubmed
Cociorva,
Validation of tandem mass spectrometry database search results using DTASelect.
2007,
Pubmed
Cohen-Cory,
Brain-derived neurotrophic factor and the development of structural neuronal connectivity.
2010,
Pubmed
Dieterich,
Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging.
2007,
Pubmed
Dieterich,
Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT).
2006,
Pubmed
Dieterich,
In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.
2010,
Pubmed
Dong,
Visual avoidance in Xenopus tadpoles is correlated with the maturation of visual responses in the optic tectum.
2009,
Pubmed
,
Xenbase
Duffy,
Long-term potentiation in the hippocampal slice: evidence for stimulated secretion of newly synthesized proteins.
1981,
Pubmed
Dziembowska,
Activity-dependent local translation of matrix metalloproteinase-9.
2012,
Pubmed
Engert,
Moving visual stimuli rapidly induce direction sensitivity of developing tectal neurons.
2002,
Pubmed
,
Xenbase
FLEXNER,
Memory in mice as affected by intracerebral puromycin.
1963,
Pubmed
Griffith,
Inhibition of calcium/calmodulin-dependent protein kinase in Drosophila disrupts behavioral plasticity.
1993,
Pubmed
Haas,
AMPA receptors regulate experience-dependent dendritic arbor growth in vivo.
2006,
Pubmed
,
Xenbase
Haas,
Targeted electroporation in Xenopus tadpoles in vivo--from single cells to the entire brain.
2002,
Pubmed
,
Xenbase
Hewapathirane,
In vivo imaging of seizure activity in a novel developmental seizure model.
2008,
Pubmed
,
Xenbase
Hinz,
Protein synthesis-dependent associative long-term memory in larval zebrafish.
2013,
Pubmed
Hinz,
Non-canonical amino acid labeling in vivo to visualize and affinity purify newly synthesized proteins in larval zebrafish.
2012,
Pubmed
Hodas,
Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT).
2012,
Pubmed
Keene,
Eukaryotic mRNPs may represent posttranscriptional operons.
2002,
Pubmed
Keleman,
Function of the Drosophila CPEB protein Orb2 in long-term courtship memory.
2007,
Pubmed
Konzer,
Stable isotope labeling in zebrafish allows in vivo monitoring of cardiac morphogenesis.
2013,
Pubmed
Liao,
Quantitative proteomic analysis of primary neurons reveals diverse changes in synaptic protein content in fmr1 knockout mice.
2008,
Pubmed
Lisman,
Mechanisms of CaMKII action in long-term potentiation.
2012,
Pubmed
Lisman,
The molecular basis of CaMKII function in synaptic and behavioural memory.
2002,
Pubmed
Liu,
Ca2+-permeable AMPA receptors in synaptic plasticity and neuronal death.
2007,
Pubmed
Malenka,
Synaptic plasticity and AMPA receptor trafficking.
2003,
Pubmed
Man,
GluA2-lacking, calcium-permeable AMPA receptors--inducers of plasticity?
2011,
Pubmed
Melemedjian,
IL-6- and NGF-induced rapid control of protein synthesis and nociceptive plasticity via convergent signaling to the eIF4F complex.
2010,
Pubmed
Mu,
Spike timing-dependent LTP/LTD mediates visual experience-dependent plasticity in a developing retinotectal system.
2006,
Pubmed
,
Xenbase
Nedivi,
Molecular analysis of developmental plasticity in neocortex.
1999,
Pubmed
Ngo,
Noncanonical amino acids in the interrogation of cellular protein synthesis.
2011,
Pubmed
Oruganty-Das,
Translational control of mitochondrial energy production mediates neuron morphogenesis.
2012,
Pubmed
Peng,
Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome.
2003,
Pubmed
Pratt,
Development and spike timing-dependent plasticity of recurrent excitation in the Xenopus optic tectum.
2008,
Pubmed
,
Xenbase
Rajan,
NMDA receptor activity stabilizes presynaptic retinotectal axons and postsynaptic optic tectal cell dendrites in vivo.
1999,
Pubmed
,
Xenbase
Richter,
Translational control of synaptic plasticity.
2010,
Pubmed
Rozov,
Facilitation of currents through rat Ca2+-permeable AMPA receptor channels by activity-dependent relief from polyamine block.
1998,
Pubmed
Sadygov,
Code developments to improve the efficiency of automated MS/MS spectra interpretation.
2002,
Pubmed
Schwartz,
Neural activity regulates synaptic properties and dendritic structure in vivo through calcineurin/NFAT signaling.
2009,
Pubmed
,
Xenbase
Schwartz,
Activity-dependent transcription of BDNF enhances visual acuity during development.
2011,
Pubmed
,
Xenbase
Shashoua,
Brain protein metabolism and the acquisition of new patterns of behavior.
1977,
Pubmed
Shashoua,
Brain protein metabolism and the acquisition of new behaviors. II. Immunological studies of the alpha, beta and gamma proteins of goldfish brain.
1977,
Pubmed
Shen,
Inhibition to excitation ratio regulates visual system responses and behavior in vivo.
2011,
Pubmed
,
Xenbase
Si,
A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia.
2003,
Pubmed
,
Xenbase
Sin,
Dendrite growth increased by visual activity requires NMDA receptor and Rho GTPases.
2002,
Pubmed
,
Xenbase
Speers,
Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)-ABPP by MudPIT Mass Spectrometry.
2009,
Pubmed
Speers,
Profiling enzyme activities in vivo using click chemistry methods.
2004,
Pubmed
Sutton,
Dendritic protein synthesis, synaptic plasticity, and memory.
2006,
Pubmed
Tabb,
DTASelect and Contrast: tools for assembling and comparing protein identifications from shotgun proteomics.
2002,
Pubmed
Villén,
The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry.
2008,
Pubmed
Washburn,
Large-scale analysis of the yeast proteome by multidimensional protein identification technology.
2001,
Pubmed
Weerapana,
Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)--a general method for mapping sites of probe modification in proteomes.
2007,
Pubmed
Wells,
A role for the cytoplasmic polyadenylation element in NMDA receptor-regulated mRNA translation in neurons.
2001,
Pubmed
West,
Neuronal activity-regulated gene transcription in synapse development and cognitive function.
2011,
Pubmed
Worley,
Synaptic regulation of immediate-early genes in brain.
1990,
Pubmed
Wu,
Maturation of a central glutamatergic synapse.
1996,
Pubmed
,
Xenbase
Wu,
CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of alpha-CaMKII mRNA at synapses.
1998,
Pubmed
,
Xenbase
Wu,
Stabilization of dendritic arbor structure in vivo by CaMKII.
1998,
Pubmed
,
Xenbase
Yang,
Comparative analysis of cleavable azobenzene-based affinity tags for bioorthogonal chemical proteomics.
2010,
Pubmed
