Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Proteomics
2018 Oct 01;1710:1991-2004. doi: 10.1074/mcp.RA118.000745.
Show Gene links
Show Anatomy links
Proteomic Profiling of Microtubule Self-organization in M-phase.
Rosas-Salvans M
,
Cavazza T
,
Espadas G
,
Sabido E
,
Vernos I
.
???displayArticle.abstract???
Microtubules (MTs) and associated proteins can self-organize into complex structures such as the bipolar spindle, a process in which RanGTP plays a major role. Addition of RanGTP to M-phase Xenopus egg extracts promotes the nucleation and self-organization of MTs into asters and bipolar-like structures in the absence of centrosomes or chromosomes. We show here that the complex proteome of these RanGTP-induced MT assemblies is similar to that of mitotic spindles. Using proteomic profiling we show that MT self-organization in the M-phase cytoplasm involves the non-linear and non-stoichiometric recruitment of proteins from specific functional groups. Our study provides for the first time a temporal understanding of the protein dynamics driving MT self-organization in M-phase.
Bonner,
Mitotic spindle proteomics in Chinese hamster ovary cells.
2011, Pubmed
Bonner,
Mitotic spindle proteomics in Chinese hamster ovary cells.
2011,
Pubmed
Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase
Caudron,
Spatial coordination of spindle assembly by chromosome-mediated signaling gradients.
2005,
Pubmed
,
Xenbase
Cavazza,
The RanGTP Pathway: From Nucleo-Cytoplasmic Transport to Spindle Assembly and Beyond.
2015,
Pubmed
Clarke,
Spatial and temporal coordination of mitosis by Ran GTPase.
2008,
Pubmed
Cortès,
Microtubule self-organisation by reaction-diffusion processes in miniature cell-sized containers and phospholipid vesicles.
2006,
Pubmed
Cox,
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification.
2008,
Pubmed
Cozza,
Quinalizarin as a potent, selective and cell-permeable inhibitor of protein kinase CK2.
2009,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Deutsch,
The ProteomeXchange consortium in 2017: supporting the cultural change in proteomics public data deposition.
2017,
Pubmed
Dinarina,
Chromatin shapes the mitotic spindle.
2009,
Pubmed
,
Xenbase
Dogterom,
Microtubule organization in vitro.
2013,
Pubmed
Franchin,
Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells.
2015,
Pubmed
Gache,
Xenopus meiotic microtubule-associated interactome.
2010,
Pubmed
,
Xenbase
Groen,
Microtubule assembly in meiotic extract requires glycogen.
2011,
Pubmed
,
Xenbase
Huang,
MiCroKiTS 4.0: a database of midbody, centrosome, kinetochore, telomere and spindle.
2015,
Pubmed
Karsenti,
Self-organization in cell biology: a brief history.
2008,
Pubmed
Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase
Kinoshita,
Reconstitution of physiological microtubule dynamics using purified components.
2001,
Pubmed
,
Xenbase
Ma,
How protein kinases co-ordinate mitosis in animal cells.
2011,
Pubmed
Manning,
The protein kinase complement of the human genome.
2002,
Pubmed
Meunier,
K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly.
2011,
Pubmed
,
Xenbase
Mitchison,
Dynamic instability of microtubule growth.
,
Pubmed
Mitchison,
Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol.
2013,
Pubmed
,
Xenbase
Nakamura,
When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to gamma-tubulin.
1998,
Pubmed
Nédélec,
Self-organisation and forces in the microtubule cytoskeleton.
2003,
Pubmed
Nédélec,
Computer simulations reveal motor properties generating stable antiparallel microtubule interactions.
2002,
Pubmed
,
Xenbase
Neumann,
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
2010,
Pubmed
Neumann,
High-throughput RNAi screening by time-lapse imaging of live human cells.
2006,
Pubmed
Oh,
Spatial organization of the Ran pathway by microtubules in mitosis.
2016,
Pubmed
Pavin,
Self-Organization and Forces in the Mitotic Spindle.
2016,
Pubmed
Petry,
Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2.
2013,
Pubmed
,
Xenbase
Petry,
Augmin promotes meiotic spindle formation and bipolarity in Xenopus egg extracts.
2011,
Pubmed
,
Xenbase
Prosser,
Mitotic spindle assembly in animal cells: a fine balancing act.
2017,
Pubmed
Rao,
The Clathrin-dependent Spindle Proteome.
2016,
Pubmed
Rappsilber,
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips.
2007,
Pubmed
Sauer,
Proteome analysis of the human mitotic spindle.
2005,
Pubmed
Schaffner,
Biophysical model of self-organized spindle formation patterns without centrosomes and kinetochores.
2006,
Pubmed
,
Xenbase
Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase
Surrey,
Physical properties determining self-organization of motors and microtubules.
2001,
Pubmed
Szklarczyk,
STRING v10: protein-protein interaction networks, integrated over the tree of life.
2015,
Pubmed
Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase
Verde,
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
1991,
Pubmed
,
Xenbase
Vignaud,
Directed cytoskeleton self-organization.
2012,
Pubmed
Wilde,
Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities.
2001,
Pubmed
,
Xenbase
Wilhelm,
Protein kinase CK2 is implicated in early steps of the differentiation of pre-adipocytes into adipocytes.
2012,
Pubmed
Wühr,
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
2014,
Pubmed
,
Xenbase
Zhang,
Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity.
2008,
Pubmed
,
Xenbase
