Figure 2. Expression of Xl-Ink4d during Xenopus laevis development. Embryos were staged according Xenopus laevis normal tables of development [27] and RNA was harvested at the indicated stages. (a) Reverse transcription PCR was performed on total RNA from staged embryos to amplify Xl-Ink4d1, cyclin-D1, Cdk4 and ODC transcripts. Trace [α32P]-dCTP was added to the reactions and the PCR products were separated by gel electrophoresis. (b) Relative quantitative real-time PCR analysis of Xl-Ink4d1 mRNA levels was performed using iQ SYBR Green Supermix (Bio-Rad), run and detected using an iCycler thermocycler (Bio-Rad). (c) In situ hybridization (ISH) for Xl-Ink4d1 expression during early embryonic development. Xl-Ink4d1 expression is detected at low levels in the dorsal anterior region of the developing tadpole. Stage 19 (iâiii); (i) dorsal view, (ii) anterior view, (iii) posterior view. Stage 22 (ivâvi); (iv) lateral view, (v) dorsal view, (vi) anterior view. Stage 32 (vii), trunk somites (S), eye (E) and head (H). (d) Immunoblotting of Xl-Ink4d from whole embryo lysates at the indicated stages using a polyclonal antibody directed against the C-terminus of the protein. Embryos microinjected with synthesized Xl-Ink4d1 mRNA were harvested and run as a positive control (+).
Image published in: Doherty JR et al. (2014)
Copyright: © 2014 Doherty JR, et al. Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cdkn2d.L | ank2, ankyrin 2, neuronal, ink4d, p19, p19-ink4d | X. laevis | Throughout NF stage 19 | dorsal neural plate |
| cdkn2d.L | ank2, ankyrin 2, neuronal, ink4d, p19, p19-ink4d | X. laevis | Throughout NF stage 22 | neural tube posterior neural tube anterior neural tube optic vesicle |
| cdkn2d.L | ank2, ankyrin 2, neuronal, ink4d, p19, p19-ink4d | X. laevis | Throughout NF stage 32 | eye head somite brain |
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